Table of Contents

Editors' Picks Highlights

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A G_s-RhoGEF interaction: An old G protein finds a new job
- Vladlen Z. Slepak and
- Alexey Pronin
J. Biol. Chem. 2020 295: 16929-. doi:10.1074/jbc.H120.016606

Editors' Picks

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Interrogation of kinase genetic interactions provides a global view of PAK1-mediated signal transduction pathways
- Jae-Hong Kim,
- Yeojin Seo,
- Myungjin Jo,
- Hyejin Jeon,
- Young-Seop Kim,
- Eun-Jung Kim,
- Donggun Seo,
- Won-Ha Lee,
- Sang Ryong Kim,
- Nozomu Yachie,
- Quan Zhong,
- Marc Vidal,
- Frederick P. Roth,
- and Kyoungho Suk
J. Biol. Chem. 2020 295: 16906-. doi:10.1074/jbc.RA120.014831
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Gα_s directly drives PDZ-RhoGEF signaling to Cdc42
- Alejandro Castillo-Kauil,
- Irving García-Jiménez,
- Rodolfo Daniel Cervantes-Villagrana,
- Sendi Rafael Adame-García,
- Yarely Mabell Beltrán-Navarro,
- J. Silvio Gutkind,
- Guadalupe Reyes-Cruz,
- and José Vázquez-Prado
J. Biol. Chem. 2020 295: 16920-. doi:10.1074/jbc.AC120.015204

JBC Reviews

author's choice

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Building better polymerases: Engineering the replication of expanded genetic alphabets
- Zahra Ouaray,
- Steven A. Benner,
- Millie M. Georgiadis,
- and Nigel G. J. Richards
J. Biol. Chem. 2020 295: 17046-. doi:10.1074/jbc.REV120.013745
AUTHOR'S CHOICE
Select this article
Post-translational regulation of the major drug transporters in the families of organic anion transporters and organic anion–transporting polypeptides
- Wooin Lee,
- Jeong-min Ha,
- and Yuichi Sugiyama
J. Biol. Chem. 2020 295: 17349-. doi:10.1074/jbc.REV120.009132

Accelerated Communications

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Mycobacteria excise DNA damage in 12- or 13-nucleotide-long oligomers by prokaryotic-type dual incisions and performs transcription-coupled repair
- Christopher P. Selby,
- Laura A. Lindsey-Boltz,
- Yanyan Yang,
- and Aziz Sancar
J. Biol. Chem. 2020 295: 17374-. doi:10.1074/jbc.AC120.016325

Cell Biology

author's choice

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Site-specific contacts enable distinct modes of TRPV1 regulation by the potassium channel Kvβ1 subunit
- Yuanyuan Wang,
- Xiaoyi Mo,
- Conghui Ping,
- Qian Huang,
- Hao Zhang,
- Chang Xie,
- Bo Zhong,
- Dongdong Li,
- and Jing Yao
J. Biol. Chem. 2020 295: 17337-. doi:10.1074/jbc.RA120.015605
AUTHOR'S CHOICE

DNA and Chromosomes

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A human cancer cell line initiates DNA replication normally in the absence of ORC5 and ORC2 proteins
- Etsuko Shibata and
- Anindya Dutta
J. Biol. Chem. 2020 295: 16949-. doi:10.1074/jbc.RA120.015450
Select this article
Nonspecific DNA binding by P1 ParA determines the distribution of plasmid partition and repressor activities
- Jamie C. Baxter,
- William G. Waples,
- and Barbara E. Funnell
J. Biol. Chem. 2020 295: 17298-. doi:10.1074/jbc.RA120.015642

Enzymology

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Enhanced enzyme kinetics of reverse transcriptase variants cloned from animals infected with SIVmac239 lacking viral protein X
- Si'Ana A. Coggins,
- Dong-Hyun Kim,
- Raymond F. Schinazi,
- Ronald C. Desrosier,
- and Baek Kim
J. Biol. Chem. 2020 295: 16975-. doi:10.1074/jbc.RA120.015273
Select this article
Mutation of an atypical oxirane oxyanion hole improves regioselectivity of the α/β-fold epoxide hydrolase Alp1U
- Liping Zhang,
- Bidhan Chandra De,
- Wenjun Zhang,
- Attila Mándi,
- Zhuangjie Fang,
- Chunfang Yang,
- Yiguang Zhu,
- Tibor Kurtán,
- and Changsheng Zhang
J. Biol. Chem. 2020 295: 16987-. doi:10.1074/jbc.RA120.015563
Select this article
Kinetic investigation of the polymerase and exonuclease activities of human DNA polymerase ε holoenzyme
- Walter J. Zahurancik and
- Zucai Suo
J. Biol. Chem. 2020 295: 17251-. doi:10.1074/jbc.RA120.013903
Select this article
Optimized incorporation of an unnatural fluorescent amino acid affords measurement of conformational dynamics governing high-fidelity DNA replication
- Tyler L. Dangerfield and
- Kenneth A. Johnson
J. Biol. Chem. 2020 295: 17265-. doi:10.1074/jbc.RA120.015557

Gene Regulation

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Genetic evidence for partial redundancy between the arginine methyltransferases CARM1 and PRMT6
- Donghang Cheng,
- Guozhen Gao,
- Alessandra Di Lorenzo,
- Sandrine Jayne,
- Michael O. Hottiger,
- Stephane Richard,
- and Mark T. Bedford
J. Biol. Chem. 2020 295: 17060-. doi:10.1074/jbc.RA120.014704
author's choice

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A novel stress-inducible CmtR-ESX3-Zn²⁺ regulatory pathway essential for survival of Mycobacterium bovis under oxidative stress
- Xiaohui Li,
- Liu Chen,
- Jingjing Liao,
- Jiechen Hui,
- Weihui Li,
- and Zheng-Guo He
J. Biol. Chem. 2020 295: 17083-. doi:10.1074/jbc.RA120.013017
AUTHOR'S CHOICE
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The glucose-sensing transcription factor ChREBP is targeted by proline hydroxylation
- Steffi Heidenreich,
- Pamela Weber,
- Heike Stephanowitz,
- Konstantin M. Petricek,
- Till Schütte,
- Moritz Oster,
- Antti M. Salo,
- Miriam Knauer,
- Isabel Goehring,
- Na Yang,
- Nicole Witte,
- Anne Schumann,
- Manuela Sommerfeld,
- Matthias Muenzner,
- Johanna Myllyharju,
- Eberhard Krause,
- and Michael Schupp
J. Biol. Chem. 2020 295: 17158-. doi:10.1074/jbc.RA120.014402
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The Arg-293 of Cryptochrome1 is responsible for the allosteric regulation of CLOCK-CRY1 binding in circadian rhythm
- Seref Gul,
- Cihan Aydin,
- Onur Ozcan,
- Berke Gurkan,
- Saliha Surme,
- Ibrahim Baris,
- and Ibrahim Halil Kavakli
J. Biol. Chem. 2020 295: 17187-. doi:10.1074/jbc.RA120.014333

Glycobiology and Extracellular Matrices

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Characterizing human α-1,6-fucosyltransferase (FUT8) substrate specificity and structural similarities with related fucosyltransferases
- Bhargavi M. Boruah,
- Renuka Kadirvelraj,
- Lin Liu,
- Annapoorani Ramiah,
- Chao Li,
- Guanghui Zong,
- Gerlof P. Bosman,
- Jeong-Yeh Yang,
- Lai-Xi Wang,
- Geert-Jan Boons,
- Zachary A. Wood,
- and Kelley W. Moremen
J. Biol. Chem. 2020 295: 17027-. doi:10.1074/jbc.RA120.014625
Select this article
Fluctuation in O-GlcNAcylation inactivates STIM1 to reduce store-operated calcium ion entry via down-regulation of Ser⁶²¹ phosphorylation
- Atsuo Nomura,
- Shunichi Yokoe,
- Kiichiro Tomoda,
- Takatoshi Nakagawa,
- Francisco Javier Martin-Romero,
- and Michio Asahi
J. Biol. Chem. 2020 295: 17071-. doi:10.1074/jbc.RA120.014271

Immunology

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A trade-off switch of two immunological memories in Caenorhabditis elegans reinfected by bacterial pathogens
- Jinyuan Yan,
- Ninghui Zhao,
- Zhongshan Yang,
- Yuhong Li,
- Hua Bai,
- Wei Zou,
- Keqin Zhang,
- and Xiaowei Huang
J. Biol. Chem. 2020 295: 17323-. doi:10.1074/jbc.RA120.013923

Membrane Biology

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pH-dependent pyridoxine transport by SLC19A2 and SLC19A3: Implications for absorption in acidic microclimates
- Takahiro Yamashiro,
- Tomoya Yasujima,
- Hamid M. Said,
- and Hiroaki Yuasa
J. Biol. Chem. 2020 295: 16998-. doi:10.1074/jbc.RA120.013610
Select this article
Functional impact of a congenital stationary night blindness type 2 mutation depends on subunit composition of Ca_v1.4 Ca²⁺ channels
- Brittany Williams,
- Josue A. Lopez,
- J. Wesley Maddox,
- and Amy Lee
J. Biol. Chem. 2020 295: 17215-. doi:10.1074/jbc.RA120.014138

Metabolism

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CDKN2A/p16INK4a suppresses hepatic fatty acid oxidation through the AMPKα2-SIRT1-PPARα signaling pathway
- Yann Deleye,
- Alexia Karen Cotte,
- Sarah Anissa Hannou,
- Nathalie Hennuyer,
- Lucie Bernard,
- Bruno Derudas,
- Sandrine Caron,
- Vanessa Legry,
- Emmanuelle Vallez,
- Emilie Dorchies,
- Nathalie Martin,
- Steve Lancel,
- Jean Sébastien Annicotte,
- Kadiombo Bantubungi,
- Albin Pourtier,
- Violeta Raverdy,
- François Pattou,
- Philippe Lefebvre,
- Corinne Abbadie,
- Bart Staels,
- Joel T. Haas,
- and Réjane Paumelle
J. Biol. Chem. 2020 295: 17310-. doi:10.1074/jbc.RA120.012543

Microbiology

author's choice

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Cholesterol sensing by CD81 is important for hepatitis C virus entry
- Machaela Palor,
- Lenka Stejskal,
- Piya Mandal,
- Annasara Lenman,
- María Pía Alberione,
- Jared Kirui,
- Rebecca Moeller,
- Stefan Ebner,
- Felix Meissner,
- Gisa Gerold,
- Adrian J. Shepherd,
- and Joe Grove
J. Biol. Chem. 2020 295: 16931-. doi:10.1074/jbc.RA120.014761
AUTHOR'S CHOICE
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Amyloid precursor protein is a restriction factor that protects against Zika virus infection in mammalian brains
- Amy Lingel,
- Haishuang Lin,
- Yuval Gavriel,
- Eric Weaver,
- Pascal Polepole,
- Virginia Lopez,
- Yuguo Lei,
- Thomas M. Petro,
- Beka Solomon,
- Chi Zhang,
- and Luwen Zhang
J. Biol. Chem. 2020 295: 17114-. doi:10.1074/jbc.RA120.015165
Select this article
Identification of a domain critical for Staphylococcus aureus LukED receptor targeting and lysis of erythrocytes
- Marilyn T. Vasquez,
- Ashira Lubkin,
- Tamara Reyes-Robles,
- Christopher J. Day,
- Keenan A. Lacey,
- Michael P. Jennings,
- and Victor J. Torres
J. Biol. Chem. 2020 295: 17241-. doi:10.1074/jbc.RA120.015757

Molecular Bases of Disease

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M8R tropomyosin mutation disrupts actin binding and filament regulation: The beginning affects the middle and end
- Alice Ward Racca,
- Michael J. Rynkiewicz,
- Nicholas LaFave,
- Anita Ghosh,
- William Lehman,
- and Jeffrey R. Moore
J. Biol. Chem. 2020 295: 17128-. doi:10.1074/jbc.RA120.014713
Select this article
Microtubule affinity–regulating kinase 4 with an Alzheimer's disease-related mutation promotes tau accumulation and exacerbates neurodegeneration
- Toshiya Oba,
- Taro Saito,
- Akiko Asada,
- Sawako Shimizu,
- Koichi M. Iijima,
- and Kanae Ando
J. Biol. Chem. 2020 295: 17138-. doi:10.1074/jbc.RA120.014420
Select this article
Dysregulation of hsa-miR-34a and hsa-miR-449a leads to overexpression of PACS-1 and loss of DNA damage response (DDR) in cervical cancer
- Mysore S. Veena,
- Santanu Raychaudhuri,
- Saroj K. Basak,
- Natarajan Venkatesan,
- Parameet Kumar,
- Roopa Biswas,
- Rita Chakrabarti,
- Jing Lu,
- Trent Su,
- Marcus Gallagher-Jones,
- Marco Morselli,
- Haiqing Fu,
- Matteo Pellegrini,
- Theodore Goldstein,
- Mirit I. Aladjem,
- Matthew B. Rettig,
- Sharon P. Wilczynski,
- Daniel Sanghoon Shin,
- and Eri S. Srivatsan
J. Biol. Chem. 2020 295: 17169-. doi:10.1074/jbc.RA120.014048
author's choice

Select this article
Unusual zwitterionic catalytic site of SARS–CoV-2 main protease revealed by neutron crystallography
- Daniel W. Kneller,
- Gwyndalyn Phillips,
- Kevin L. Weiss,
- Swati Pant,
- Qiu Zhang,
- Hugh M. O'Neill,
- Leighton Coates,
- and Andrey Kovalevsky
J. Biol. Chem. 2020 295: 17365-. doi:10.1074/jbc.AC120.016154
AUTHOR'S CHOICE

Neurobiology

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Carnosine synthase deficiency is compatible with normal skeletal muscle and olfactory function but causes reduced olfactory sensitivity in aging mice
- Lihua Wang-Eckhardt,
- Asisa Bastian,
- Tobias Bruegmann,
- Philipp Sasse,
- and Matthias Eckhardt
J. Biol. Chem. 2020 295: 17100-. doi:10.1074/jbc.RA120.014188

Protein Structure and Folding

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Representative cancer-associated U2AF2 mutations alter RNA interactions and splicing
- Debanjana Maji,
- Eliezra Glasser,
- Steven Henderson,
- Justin Galardi,
- Mary J. Pulvino,
- Jermaine L. Jenkins,
- and Clara L. Kielkopf
J. Biol. Chem. 2020 295: 17148-. doi:10.1074/jbc.RA120.015339

Protein Synthesis and Degradation

author's choice

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Stop codon read-through of mammalian MTCH2 leading to an unstable isoform regulates mitochondrial membrane potential
- Lekha E. Manjunath,
- Anumeha Singh,
- Sarthak Sahoo,
- Ashutosh Mishra,
- Jinsha Padmarajan,
- Chaithanya G. Basavaraju,
- and Sandeep M. Eswarappa
J. Biol. Chem. 2020 295: 17009-. doi:10.1074/jbc.RA120.014253
AUTHOR'S CHOICE
Select this article
Heme oxygenase-2 is post-translationally regulated by heme occupancy in the catalytic site
- Liu Liu,
- Arti B. Dumbrepatil,
- Angela S. Fleischhacker,
- E. Neil G. Marsh,
- and Stephen W. Ragsdale
J. Biol. Chem. 2020 295: 17227-. doi:10.1074/jbc.RA120.014919

RNA

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Iron-mediated degradation of ribosomes under oxidative stress is attenuated by manganese
- Daniel G. J. Smethurst,
- Nikolay Kovalev,
- Erica R. McKenzie,
- Dimitri G. Pestov,
- and Natalia Shcherbik
J. Biol. Chem. 2020 295: 17200-. doi:10.1074/jbc.RA120.015025

Signal Transduction

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The heptameric structure of the flagellar regulatory protein FlrC is indispensable for ATPase activity and disassembled by cyclic-di-GMP
- Shrestha Chakraborty,
- Maitree Biswas,
- Sanjay Dey,
- Shubhangi Agarwal,
- Tulika Chakrabortty,
- Biplab Ghosh,
- and Jhimli Dasgupta
J. Biol. Chem. 2020 295: 16960-. doi:10.1074/jbc.RA120.014083
Select this article
Leptin modulates pancreatic β-cell membrane potential through Src kinase–mediated phosphorylation of NMDA receptors
- Veronica A. Cochrane,
- Yi Wu,
- Zhongying Yang,
- Assmaa ElSheikh,
- Jeremy Dunford,
- Paul Kievit,
- Dale A. Fortin,
- and Show-Ling Shyng
J. Biol. Chem. 2020 295: 17281-. doi:10.1074/jbc.RA120.015489

Additions and Corrections

Correction: Functional domain and motif analyses of androgen receptor coregulator ARA70 and its differential expression in prostate cancer.
- Yueh-Chiang Hu,
- Shuyuan Yeh,
- Shauh-Der Yeh,
- Erik R. Sampson,
- Jiaoti Huang,
- Peng Li,
- Cheng-Lung Hsu,
- Huei-Ju Ting,
- Hui-Kuan Lin,
- Liang Wang,
- Eungseok Kim,
- Jing Ni,
- and Chawnshang Chang
J. Biol. Chem. 2020 295: 17382. doi:10.1074/jbc.AAC120.016763

Withdrawals/Retractions

Withdrawal: FGF16 promotes invasive behavior of SKOV-3 ovarian cancer cells through activation of mitogen-activated protein kinase (MAPK) signaling pathway.
- Moitri Basu,
- Satinath Mukhopadhyay,
- Uttara Chatterjee,
- and Sib Sankar Roy
J. Biol. Chem. 2020 295: 17381. doi:10.1074/jbc.W120.016735

Editors' Picks Highlights

A Gs-RhoGEF interaction: An old G protein finds a new job

Editors' Picks

Interrogation of kinase genetic interactions provides a global view of PAK1-mediated signal transduction pathways

Gαs directly drives PDZ-RhoGEF signaling to Cdc42

JBC Reviews

Building better polymerases: Engineering the replication of expanded genetic alphabets

Post-translational regulation of the major drug transporters in the families of organic anion transporters and organic anion–transporting polypeptides

Accelerated Communications

Mycobacteria excise DNA damage in 12- or 13-nucleotide-long oligomers by prokaryotic-type dual incisions and performs transcription-coupled repair

Cell Biology

Site-specific contacts enable distinct modes of TRPV1 regulation by the potassium channel Kvβ1 subunit

DNA and Chromosomes

A human cancer cell line initiates DNA replication normally in the absence of ORC5 and ORC2 proteins

Nonspecific DNA binding by P1 ParA determines the distribution of plasmid partition and repressor activities

Enzymology

Enhanced enzyme kinetics of reverse transcriptase variants cloned from animals infected with SIVmac239 lacking viral protein X

Mutation of an atypical oxirane oxyanion hole improves regioselectivity of the α/β-fold epoxide hydrolase Alp1U

Kinetic investigation of the polymerase and exonuclease activities of human DNA polymerase ε holoenzyme

Optimized incorporation of an unnatural fluorescent amino acid affords measurement of conformational dynamics governing high-fidelity DNA replication

Gene Regulation

Genetic evidence for partial redundancy between the arginine methyltransferases CARM1 and PRMT6

A novel stress-inducible CmtR-ESX3-Zn2+ regulatory pathway essential for survival of Mycobacterium bovis under oxidative stress

The glucose-sensing transcription factor ChREBP is targeted by proline hydroxylation

The Arg-293 of Cryptochrome1 is responsible for the allosteric regulation of CLOCK-CRY1 binding in circadian rhythm

Glycobiology and Extracellular Matrices

Characterizing human α-1,6-fucosyltransferase (FUT8) substrate specificity and structural similarities with related fucosyltransferases

Fluctuation in O-GlcNAcylation inactivates STIM1 to reduce store-operated calcium ion entry via down-regulation of Ser621 phosphorylation

Immunology

A trade-off switch of two immunological memories in Caenorhabditis elegans reinfected by bacterial pathogens

Membrane Biology

pH-dependent pyridoxine transport by SLC19A2 and SLC19A3: Implications for absorption in acidic microclimates

Functional impact of a congenital stationary night blindness type 2 mutation depends on subunit composition of Cav1.4 Ca2+ channels

Metabolism

CDKN2A/p16INK4a suppresses hepatic fatty acid oxidation through the AMPKα2-SIRT1-PPARα signaling pathway

Microbiology

Cholesterol sensing by CD81 is important for hepatitis C virus entry

Amyloid precursor protein is a restriction factor that protects against Zika virus infection in mammalian brains

Identification of a domain critical for Staphylococcus aureus LukED receptor targeting and lysis of erythrocytes

Molecular Bases of Disease

M8R tropomyosin mutation disrupts actin binding and filament regulation: The beginning affects the middle and end

Microtubule affinity–regulating kinase 4 with an Alzheimer's disease-related mutation promotes tau accumulation and exacerbates neurodegeneration

Dysregulation of hsa-miR-34a and hsa-miR-449a leads to overexpression of PACS-1 and loss of DNA damage response (DDR) in cervical cancer

Unusual zwitterionic catalytic site of SARS–CoV-2 main protease revealed by neutron crystallography

Neurobiology

Carnosine synthase deficiency is compatible with normal skeletal muscle and olfactory function but causes reduced olfactory sensitivity in aging mice

Protein Structure and Folding

Representative cancer-associated U2AF2 mutations alter RNA interactions and splicing

Protein Synthesis and Degradation

Stop codon read-through of mammalian MTCH2 leading to an unstable isoform regulates mitochondrial membrane potential

Heme oxygenase-2 is post-translationally regulated by heme occupancy in the catalytic site

RNA

Iron-mediated degradation of ribosomes under oxidative stress is attenuated by manganese

Signal Transduction

The heptameric structure of the flagellar regulatory protein FlrC is indispensable for ATPase activity and disassembled by cyclic-di-GMP

Leptin modulates pancreatic β-cell membrane potential through Src kinase–mediated phosphorylation of NMDA receptors

Additions and Corrections

Correction: Functional domain and motif analyses of androgen receptor coregulator ARA70 and its differential expression in prostate cancer.

Withdrawals/Retractions

Withdrawal: FGF16 promotes invasive behavior of SKOV-3 ovarian cancer cells through activation of mitogen-activated protein kinase (MAPK) signaling pathway.

This Issue

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A G_s-RhoGEF interaction: An old G protein finds a new job

Gα_s directly drives PDZ-RhoGEF signaling to Cdc42

A novel stress-inducible CmtR-ESX3-Zn²⁺ regulatory pathway essential for survival of Mycobacterium bovis under oxidative stress

Fluctuation in O-GlcNAcylation inactivates STIM1 to reduce store-operated calcium ion entry via down-regulation of Ser⁶²¹ phosphorylation

Functional impact of a congenital stationary night blindness type 2 mutation depends on subunit composition of Ca_v1.4 Ca²⁺ channels